Heycarbons Activated Carbon For Nucleotides Decolorization Manufacturer From China
- Over 20 years of industry experience in wood activated carbon manufacturing.
- Annual output 18000 tons
- 16pcs Production Patents.
- Low S, Low Fe, High methylene blue, High caramel value.
- Popular in the activated carbon market for nucleotides decolorization.
What Are Nucleotides?
Nucleotides are basic compounds composed of a sugar (ribose or deoxyribose) + phosphate group + nitrogenous base, and are the fundamental units of DNA and RNA.
Their chemical structure includes:
- One pentose sugar (Ribose/Deoxyribose)
- One nitrogenous base (A, G, C, T, U)
- One to three phosphate groups
Nucleotides are among the most fundamental and important molecules in living systems.
Applications of Nucleotides
Nucleotides have wide applications in the following scenarios:
- Food Industry: Flavor enhancers (IMP/GMP) are used in seasonings, meat products, and convenience foods.
- Pharmaceutical Industry: Antiviral drug precursors, cell repair, and immune enhancement
- Animal Nutrition: Boost immunity and growth rate
- Fermentation Industry and Biotechnology: Fermentation cofactors, energy molecules (ATP), culture medium components
What Can Activated Carbon Do for Nucleotides?
The mother liquor following nucleotide fermentation or chemical synthesis often contains:
- Pigmentary impurities produced during fermentation (such as polyphenols and tryptophan derivatives);
- Colored complexes formed from decomposition products and metal ions;
- Protein and polysaccharide impurities.
These impurities can cause the solution to appear yellowish-brown or even dark brown, affecting the color, purity, and sensory quality of the nucleotide crystals.
Therefore, decolorization with activated carbon is necessary before crystallization.
Adsorption Principle of Activated Carbon For Nucleotide Decolorization
Activated carbon can be used for nucleotide decolorization primarily based on the following two fundamental principles:
1. Physical Adsorption
Activated carbon utilizes its large specific surface area and highly developed pore structure (mainly micropores and mesopores).
These pores can effectively adsorb colored impurities in solution through van der Waals forces. These impurities are typically large organic molecules, such as pigments, phenols, tannins, and other unknown dark-colored degradation products.
Nucleotide molecules themselves are relatively small, and under suitable conditions, the amount adsorbed is much smaller than that of large colored impurities.
2. Chemical Adsorption
The surface of activated carbon contains various functional groups (such as carboxyl groups, phenolic hydroxyl groups, carbonyl groups, etc.), giving it a surface charge.
The pH value of the solution affects the charge properties of nucleotides (which carry phosphate groups and are negatively charged) and the activated carbon surface. By adjusting the pH, the electrostatic interaction between them can be controlled, thereby reducing the loss of the target nucleotides. Acid-washed activated carbon has a stronger adsorption capacity for polar impurities.
3. Metal Ion Adsorption
Some colored metal ions (Fe, Cu, etc.) may affect the color of nucleotides.
Using acid-washed carbon allows for better control of metal ion content.
Activated Carbon For Nucleotide Decolorization Process
Activated carbon is commonly used for decolorization (removing pigment impurities) in the production of nucleotides (such as AMP, GMP, CMP, UMP, etc.).
This is a crucial step in the nucleotide purification process. The specific industrial decolorization process is as follows:
1. Equipment and Material Preparation
Reaction Vessel
o Material: 316L stainless steel, ensuring corrosion resistance (especially under acidic pH conditions) and easy cleaning.
o Design: Jacketed for hot or cold water flow to control temperature. Equipped with an explosion-proof variable frequency mechanical stirrer to ensure uniform solid-liquid mixing.
o Accessories: Equipped with an online pH meter, temperature sensor, feed port, sight glass, and bottom discharge valve.
Activated Carbon
o Specifications: Pharmaceutical-grade (or food-grade) powdered activated carbon, with a supplier’s Certificate of Quality As (CoA).
o Pretreatment: Establish standard operating procedures (SOPs) for activated carbon acceptance and pretreatment. If acid washing is not required, directly prepare a slurry of a certain concentration using water for injection (WFI) or purified water for easy pumping and dispersion.
Solution Preparation System
o Use a mixing tank with a stirrer to pre-dissolve the crude nucleotides and adjust them to the target concentration and pH range.
2. Industrial Production Process and Control Parameters
Step 1: Sample Loading Solution Preparation and Transfer
• Operation: In the mixing tank, dissolve the crude nucleotides in purified water, controlling the concentration at 5-15% (w/v) (optimize according to specific products). Use jacket cooling to prevent nucleotide degradation due to excessive temperature.
• Key Control Point 1 – pH Adjustment:
o Setpoint: Use food-grade acid (such as hydrochloric acid, citric acid) to precisely adjust and stabilize the solution pH within the range of 3.0-4.0.
o Control Method: Monitor in real-time using an online pH meter, linked to an automatic acid addition system, to ensure pH stability.
• Operation: Transfer the pH-adjusted sample loading solution to the decolorization reaction tank using a sanitary pump.
Step 2: Activated Carbon Addition and Mixing
• Critical Control Point 2 – Activated Carbon Slurry Addition:
o Setpoint: Add activated carbon at a rate of 0.3–1.0% (w/w) to the nucleotide fermentation broth or semi-finished product solution. The optimal addition amount needs to be determined through prior process validation.
o Control Method: Use a precise metering pump to add the pre-prepared activated carbon slurry to the reaction vessel through the feed port.
• Critical Control Point 3 – Temperature and Time:
o Setpoint: Control the temperature at 40 – 55°C using the reaction vessel jacket. This temperature helps improve the adsorption rate but must be below the degradation temperature of nucleotides.
o Setpoint: Start stirring, controlling the speed to ensure uniform mixing without excessive foaming, and continue stirring for 30 – 45 minutes.
o Control Method: Use a timer and start timing from the beginning of the reaction. The temperature and stirring speed are set and recorded on the control panel.
Step 3: Solid-Liquid Separation and Washing
• Procedure: After decolorization, immediately pump the mixture through the bottom discharge valve into a plate and frame filter or a horizontal plate filter.
• Critical Control Point 4 – Filtration:
o Objective: To obtain a clear and transparent filtrate.
o Control Method: The initially turbid filtrate should be returned to the front end of the filter for re-filtration until the filtrate is clear. Record the filtration rate and pressure difference.
• Critical Control Point 5 – Washing:
o Procedure: After filtration, wash the filter cake with 1-2 times the volume of activated carbon, preheated to the same temperature and with a pH of 3.0-4.0 using purified water.
o Purpose: To recover nucleotides trapped in the voids of the activated carbon cake. This step can significantly improve product yield.
o Control Method: The washing liquid can be combined with the main filtrate, or they can be collected separately according to purity requirements.
3. Post-processing and Record Keeping
• Filtrate Treatment: The collected decolorized filtrate should be transferred to the next purification process (such as ion exchange column chromatography, concentration, crystallization, etc.) as soon as possible to prevent microbial growth.
• Activated Carbon Waste Treatment: Collect the waste carbon and treat it in accordance with the company’s environmental protection regulations.
Process Record Keeping and Release:
Batch Production Record (BPR): Record in detail the operation time, pH value, temperature, activated carbon batch number, amount added, stirring time, operator, etc. for each step.
Quality Inspection: Sample the decolorized filtrate for testing. Key indicators include:
- Transmittance: Measured at a specific wavelength (e.g., 260nm), must be >95% or meet internal control standards.
- Nucleotide Content: Detected by HPLC, calculate the decolorization yield.
- pH Value and Conductivity: Meet the feed requirements for the next process.
Activated Carbon For Nucleotide Decolorization Product
Wood Powdered Activated Carbon For Nucleotide Decolorization
HEYSORBCARB-WPAC uses high-quality wood as raw material and features medium to large pore size, large adsorption capacity, and fast filtration speed.
The parameters of wood powder activated carbon are as follows:
- Particle size <200mesh (0.75μm)>: 95% min.
- Iodine value: 900-1100 mg/g
- Methylene blue value: 210-320 mg/g
- Caramel value: 100-150%
- Bulk density: 0.35-0.45 g/cm3
- Moisture: 10% max.
- Ash: ≤6%
- Ash: ≤2%（Special treatment）Metal ions (Fe, Cu): Extremely low
- PH:3-5
- Can be customized according to customer’s specific application and requirements by Heycarbons
If you want to more information about wood activated carbon, please read the article below:
Advantages of Activated Carbon for Nucleotide Decolorization
- High decolorization efficiency – strong mesoporous structure effectively captures pigments
- Fast filtration speed – reduces processing time
- High purity, low metal content – suitable for food-grade nucleotides
- High cost-effectiveness – superior to resin and membrane methods
- Does not alter the nucleotide molecular structure
Custom Heycarbons Activated Carbon Nucleotide Decolorization Solution
Heycarbons provides a full range of activated solutions at competitive prices.
Customize Heycarbons Activated Carbon Nucleotide Decolorization
Heycarbons has proudly served the gold extraction activated carbon industry with high-quality products since 2005, Heycarb can customize activated carbon for your nucleotide decolorization project.
- Heycarbons customized package to promote your brand, and free design service.
- Customized size, CTC, iodine value, ash, methylene blue, caramel, PH,etc.
- Each batch gold extraction carbon of multiple mixed testing to ensure quality. It also supports any form of third-party testing for customers.
Call Us
+86-180 3788 5195
Our Email
info@heycarbons.com
Steps to Custom Activated Carbon For Nucleotide Decolorization
Consultation
By understanding your needs and requirements, our salesmen work with you to submit the appropriate activated carbon solution.
Quotation
Heycarbons expert customer service will provide you with a free quote based on your requirements as well as product specifications and quantities.
Production
Heycarbons has sufficient inventory and strong production capacity, and will report production progress to you from time to time.
Shipping
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